CD45RO Fab-Strep, human

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Low affinity Fab fragment fused to a Twin-Strep-tag® and specific for human CD45RO
CD45RO Fab-Strep, human

CD45RO Fab-Strep is a recombinantly expressed low affinity Fab fragment fused to a Twin-Strep-tag®. Fab-Streps were developed for Traceless Affinity Cell Selection (Fab-TACS®), which is based on our Strep-tag® technology. Due to their low affinity for the target, an increase in avidity is required to ensure sufficient binding to the cell surface. Combining Fab-Streps with a Strep-Tactin® backbone leads to the formation of complexes that stably bind to cells.

Depending on the Strep-Tactin® backbone, the following applications are possible:

  • Fluorescent cell staining & sorting: Fab-Streps are combined with fluorophore-conjugated Strep-Tactin®.
  • Magnetic cell isolation: Fab-Streps are combined with Strep-Tactin® Magnetic Microbeads.

By addition of biotin, the labeling reagents dissociate mildly and rapidly from the selected target cells in all applications, yielding minimally manipulated cells for unbiased further use.

Specifications

  • Endotoxin Level: ≤ 0.1 EU/µg by LAL test
  • Form: Lyophilized
  • Possible Application: Fluorescent cell staining & sorting, magnetic cell isolation
  • Purity: ≥ 90%
  • Reconstitution: Reconstitute in 1 ml buffer, e.g. 1x PBS containing 1 mM EDTA and 0.5% BSA, for a final concentration of 50 µg/ml.
  • Specificity: Human CD45RO

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