Fab-TACS® - Traceless Affinity Cell Selection - is a positive cell isolation approach based on our Strep-tag® technology. CD8 Fab-TACS® Agarose Column Starter Kit allows label-free isolation of murine cytotoxic CD8+ T cells from splenocytes or other cell suspensions. CD8 is a co-receptor that associates with the T cell receptor and interacts with MHC class I molecules.
Strep-Tactin® TACS Agarose columns are filled with Strep-Tactin® coated agarose macrobeads developed for cell isolation. Low affinity Fab fragments fused to a Twin-Strep-tag® (Fab-Streps) bind to the matrix. Target cells adhere to the matrix based on the exclusive binding of the Fab-Strep to a surface marker. Non-target cells are efficiently washed away. In a final step, the addition of biotin causes the elution of the target cells and the Fab-Streps. After elution, the Fab-Streps self-dissociate from the cell surfaces due to their low affinity. The label-free authentic target cells are now ready for further downstream applications.
Advantages:
- Highly pure cells
- Reversible binding to the target
- Highly viable, non-activated cells
- Label-free cells due to low affinity Fab fragments
The kit contains:
- Strep-Tactin® TACS Agarose columns, 0.3 ml (2x)
- CD8 Fab-Strep, mouse
- 10x Buffer CI
- Biotin stock solution
- TACS Column Adapter
Specifications
- Capacity: 1 x 10^8 total/2x10^7 target cells per column
