Competent E. coli TOP10

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Chemically competent cells for plasmid propagation
Competent E. coli TOP10

Competent E. coli TOP10 cells are ready for heat shock transformation with vector DNA and its subsequent propagation for cloning and transfection purposes. The chemically competent cells are provided as 20 separate one-shot reactions. Transformed cells can be selected by blue/white screening.

The genotype of Competent E. coli TOP10 cells is F- mcrA (mrr-hsdRMS-mcrBC) 80lacZ M15 lacX74 recA1 ara 139 (ara-leu)7697 galU galK rpsL (StrR) endA1 nupG.

Specifications

  • Biosafety Level: S1 (Germany), BSL-2 (USA)
  • Form: Cryopreserved
  • Possible Application: Expression host for recombinat Strep-tag®II and Twin-Strep-tag® proteins

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