MEXi-293E cells

MEXi-293E cells are human embryonic kidney (HEK) cells derived from the 293 cell line and used for propagation of plasmids and expression of recombinant proteins in mammalian cells. The cell line expresses the EBNA-1 protein for episomal replication of oriP-harboring plasmids (pDSG-IBA vectors), is adapted for growth in suspension, and for use with the MEXi cell culture medium. The cells are part of the MEXi mammalian expression system.

The MEXi system was developed to offer an affordable mammalian protein expression system coming along with all essential components:

• MEXi-293E cell line
• A variety of pDSG expression vectors
• MEXi-CM culture medium
• MEXi-TM transfection medium

Further advantages:

• Transient expression for time- and cost-efficient protein production
• Multiple expression vectors with different features
• Optimized for Twin-Strep-tag®:Strep-Tactin®XT system for highly pure proteins
• IP-free system