pASG-IBA162 vector

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Cytoplasmic E. coli expression vector encoding an N-terminal FLAG-tag and C-terminal Twin-Strep-tag®
pASG-IBA162 vector

The pASG-IBA162 vector is designed for expression of recombinant proteins with an N-terminal FLAG-tag and C-terminal Twin-Strep-tag® in E. coli. The vector carries an ampicillin resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number,and the inducible tetracycline promoter/operator for the regulated expression of proteins. The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. Please note that cloning into pASG-IBA Acceptor Vectors compulsorily requires the restriction enzyme Esp3I since no other MCS for the integration of a gene of interest is available. In addition to the direct cloning of the gene of interest into pASG-IBA vectors with Esp3I, another option via a so-called Entry Vector is possible.

Specifications

  • Affinity Tag C'-terminal: Twin-Strep-tag®
  • Affinity Tag N'-terminal: FLAG
  • Cloning Method: Direct cloning using restriction enzyme Esp3I
  • Concentration: 250 ng/µl
  • Expression Host: E. coli
  • Form: Suspension in TE buffer
  • Possible Application: Vector for recombinant expression in E. coli
  • Promoter: tet Promoter
  • Resistance: Ampicillin
  • Sequence: See Documents
  • Size: 3976 bp

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