pASK-IBA3C vector
IBA Lifescience Kontakt z doradcą
pASK-IBA3C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tag®II fused to the C-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), and the inducible tetracycline promoter/operator for the regulated expression of proteins.
The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. The recombinant protein will be localized in the cytoplasm.
Specifications
- Affinity Tag C'-terminal: Strep-tag®II
- Cloning Method: Direct cloning using e.g. restriction enzyme BsaI in MCS
- Concentration: 250 ng/µl
- Expression Host: E. coli
- Form: Suspension in TE buffer
- Possible Application: Expression plasmid for Strep-tag®II fusion proteins
- Promoter: tet Promoter
- Resistance: Chloramphenicol
- Sequence: See Documents
- Size: 3001 bp
