pASK-IBA6C vector

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Periplasmic E. coli expression vector encoding an N-terminal Strep-tag®II, chloramphenicol resistance and protease cleavage site
pASK-IBA6C vector

pASK-IBA6C is a classic cloning vector devised for E. coli expression of recombinant proteins with the Strep-tag®II fused to the N-terminus. It carries the chloramphenicol resistance cassette for selection of transformed E. coli cells, F1 and ColE1 origin for a high plasmid copy number, a multiple cloning site with recognition sites for several common restriction enzymes (e.g. EcoRI, SmaI, BamHI, SalI, PstI), the inducible tetracycline promoter/operator for the regulated expression of proteins, the ompA signal for periplasmic secretion of the recombinant protein, and the sequence for a protease cleavage site.

The vector can be combined with any E. coli strain since the tet-promoter works independently of the genetic background of E. coli. Expression of the recombinant protein is induced by addition of anhydrotetracycline, which is a derivative of tetracycline that does not exhibit antibiotic activity. During the secretion into the periplasmic space the ompA signal sequence will be cleaved off. The N-terminal Strep-tag®II can be removed by the protease factor Xa.

Specifications

  • Affinity Tag N'-terminal: Strep-tag®II
  • Cloning Method: Direct cloning using e.g. restriction enzyme BsaI in MCS
  • Concentration: 250 ng/µl
  • Expression Host: E. coli
  • Form: Suspension in TE buffer
  • Possible Application: Expression plasmid for Strep-tag®II fusion proteins
  • Promoter: tet Promoter
  • Resistance: Chloramphenicol
  • Sequence: See Documents
  • Size: 3081 bp
  • Specificity: Factor Xa cleavage site

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