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Hygromycin B Gold

Hygromycin B Gold™ is an ultrapure Hygromycin B.
Hygromycin B is an aminoglycoside antibiotic produced by Streptomyces hygroscopicus. It kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis.

Numer kat. Produkt Opakowanie Opis
ant-hg-1 Hygromycin B Gold (solution) 1 g (10 x 1 ml) Selective antibiotic for the hph gene
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ant-hg-5 Hygromycin B Gold (solution) 5 g (1 x 50 ml) Selective antibiotic for the hph gene
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OPIS

Resistance to Hygromycin B

Hygromycin B is used as a selective agent in molecular genetics experiments on a wide variety of eukaryotic and prokaryotic species [3-5].
The hph gene confers hygromycin-resistance to cells expressing it and many vectors carrying the hph gene are available within the scientific community. Typically, mammalian cells are sensitive to Hygromycin B concentrations of 50-200 µg/ml, and bacteria to 50-100 µg/ml.

Description:

The working concentrations of Hygromycin B Gold for mammalian cell lines vary from 50 to 200 μg/ml, in a few cases up to 500 μg/ml.
If you notice unusually high cytotoxicity when switching to Hygromycin B Gold, you may need to adjust your working concentrations.

Specifcations:

Product concentration: 100 mg/ml solution
CAS number: 31282-04-9

Quality Control: Each lot is thoroughly tested to ensure the absence of lot-to-lot variation.
Purity: ≥ 90% (HPLC)
Endotoxin level: < 0.5 EU/mg
Physicochemical characterization: pH, appearance
Cell culture tested: potency validated in hygromycin‑sensitive and hygromycin-resistant mammalian cell lines
Non‑cytotoxicity of trace contaminants: absence of long‑term effects confirmed in hygromycin‑resistant cells

 

Hygromycin B Gold™ – Manufacturer’s Website

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