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PlasmoTest™ – Mycoplasma Detection Kit

PlasmoTest™ provides a simple, rapid and reliable assay for the visual detection of mycoplasma contamination in cell cultures. This assay is the first to utilize cells to signal the presence of mycoplasma.

Numer kat. Produkt Opakowanie Opis
rep-pt1 PlasmoTest™ 1 kit (250 samples) Mycoplasma contamination detection kit
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OPIS

The Mycoplasma sensor cells detect the presence of mycoplasmas leading to a color change of the HEK-Blue™ Detection medium. The Mycoplasma sensor cells recognize mycoplasmas through Toll-like Receptor 2 (TLR2), a pathogen recognition receptor. In the presence of mycoplasmas, TLR2 initiates a signaling cascade leading to the activation of NF-kB and other transcription factors. These transcription factors induce the secretion of SEAP (secreted embryonic alkaline phosphatase) in the supernatant which is readily detected by the purple/blue coloration of the HEK-Blue™ Detection medium.

  • Requires only basic cell culture knowledge
  • Hands-on time less than 1 hour. Gives results after overnight incubation
  • Detects all cell culture contaminants
  • Detects 5.102-5.105 cfu/ml mycoplasmas
  • No false positive: a positive result indicates the presence of a cell culture contaminant

PlasmoTest™ Kit (#rep-pt1) 250 samples:
– HEK-Blue™-2 Cells: 1 vial of 3-5 x 106 frozen cells. Shipped in dry ice
– HEK-Blue™ Selection: 2 x 1 ml of 250X solution of selective antibiotics.
– Normocin™: 1 ml of 500X Normocin™ (1 ml for 500 ml of cell culture medium)
– HEK-Blue™ Detection: 1 pouch (for 50 ml of reconstituted detection medium each)
– HEK-Blue™ Water: 60 ml bottle
– Positive control: 1 tube
– Negative control: 1 tube

 

PlasmoTest™ – Mycoplasma Detection Kit – Producent Website

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