10x Buffer BXT

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Strep-Tactin®XT elution buffer
10x Buffer BXT

10x Buffer BXT is a 10x concentrated Strep-Tactin®XT elution buffer containing biotin. Biotin is a water-soluble B-complex vitamin, also known as vitamin B7 or vitamin H. It binds to Strep-Tactin®XT with very high affinity, but reversibly, and thereby allows the efficient elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from the resin. Please note that 10x Buffer BXT is not applicable for elution of Strep-tag®II or Twin-Strep-tag® fusion proteins from Strep-Tactin®, since biotin irreversibly binds to these resins, and thus Strep-Tactin® cannot be regenerated.

Specifications

  • Concentration: 10x concentrated
  • Form: Solution
  • Possible Application: Competitive elution of Strep-tag®II and Twin-Strep-tag® fusion proteins from Strep-Tactin®XT resins

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