Strep-Tactin® Superflow® high capacity column

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Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin® Superflow® high capacity column

Strep-Tactin® Superflow® high capacity columns contain a 6% agarose coupled with the streptavidin variant Strep-Tactin® and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. In comparison to Strep-Tactin® Superflow®, Strep-Tactin® Superflow® high capacity has a higher density of immobilized Strep-Tactin®, which leads to a 3- to 5-fold higher yield of purified protein.

To reduce the waiting period during sample and buffer application, the use of the application aid WET FRED is recommended.

Specifications

  • Bead Size: 60 - 160 µm spherical
  • Biotin Binding Capacity: > 900 nmol/ml resin
  • Dynamic Protein Binding Capacity: 7 mg/ml resin
  • Exclusion Limit: 6 x 10^6
  • Form: Pre-packed gravity flow column
  • Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
  • Specificity: Twin-Strep-Tag®; Strep-tag®II; cross reactivity: biotin
  • Support: 6% agarose, crosslinked

Videos

Color based functionality check - Strep-Tactin® and Strep-Tactin®XT columns compared

Protein Purification with Large Sample Volumes using Wet Fred

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