Strep-Tactin®XT 4Flow® column

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Gravity flow column to purify Strep-tag®II or Twin-Strep-tag® fusion proteins
Strep-Tactin®XT 4Flow® column

Strep-Tactin®XT 4Flow® columns contain a 4% agarose coupled with the streptavidin variant Strep-Tactin®XT and are applicable for purification of Strep-tag®II or Twin-Strep-tag® fusion proteins via gravity flow. Due to the broad pH compatibility, excellent pressure stability, outstanding target purity (> 95%), and highest binding affinity, the resin is recommended for all protein classes, especially for low abundant or challenging proteins. The low concentrated agarose allows for the simple attachment of larger proteins to the stationary phase, making it particularly suitable for purification of high-molecular-weight targets. In addition, Strep-Tactin®XT 4Flow® enables the application of denaturing agents such as urea.

To reduce the waiting period during the sample and buffer application, the use of the application aid WET FRED is recommended.

Specifications

  • Bead Size: 50 - 150 µm spherical
  • Dynamic Protein Binding Capacity: 5 mg/ml resin
  • Form: Pre-packed gravity flow column
  • Possible Application: Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins
  • Specificity: Twin-Strep-Tag® and Strep-tag®II
  • Support: 4% agarose, crosslinked

Videos

Protein Purification Tutorial with Strep-Tactin®XT 4Flow®

Strep-Tactin®XT 4Flow® Color based functionality check

Protein Purification with Large Sample Volumes using Wet Fred

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