5’ triphosphate double-stranded RNA (5’ppp-dsRNA) is a synthetic ligand for the retinoic acid-inducible protein I (RIG-I, also known as Ddx58). RIG-I is a cytoplasmic RNA helicase that belongs to the RIG-I-like receptors (RLRs) family and triggers an antiviral immune response by the activation of type-I interferons (IFN-α and -β) . RIG-I detects viral RNAs that exhibit an uncapped 5’-di/triphosphate end and a short blunt-ended double-strand portion, two essential features facilitating discrimination from self-RNAs [2-4].
To achieve stimulation of RIG-I, 5’ppp-dsRNA must be delivered into the cytoplasm, for example by using a transfection agent, such as LyoVec™.
Key features of 5'ppp-dsRNA:
- 5'ppp-dsRNA is a specific RIG-I agonist.
- Its biological activity has been verified using our RLR reporter cell lines.
- For your convenience, pre-complexed 5'ppp-dsRNA/ LyoVec™ is also available.
1. Yoneyama M. & Fujita T. , 2007. Function of RIG-I-like Receptors in Antiviral Innate Immunity. J. Biol. Chem. 282:15315-8. 2. Schlee M. et al., 2009. Recognition of 5’ triphosphate by RIG-I helicase requires short blunt double-stranded RNA as contained in pandhandle of negative-strand virus. Immunity. 17;31(1):25-34. 3. Schmidt A. et al., 2009. 5’-triphosphate RNA requires base-paired structures to activate antiviral signaling via RIG-I. PNAS 106(29):12067-72. 4. Schlee M. & Hartmann G., 2010. The chase for the RIG-I ligand - recent advances. Mol Ther. 18(7):1254-62.