Pfu DNA polymerase 1U/µl
Biotools Kontakt z doradcąHigh-fidelity DNA Polymerase from Pyrococcus furiosus, cloned in E.coli. with proofreading (3'-5' exonuclease) activity and a reduced error rate (10-fold lower than standard Taq DNA Polymerases).
Biotools Pfu DNA Polymerase generates blunt-ended amplification products, enabling their cloning into blunt-ended vectors.
It is suitable for applications including:
- Cloning
- Mutagenesis
- Achieving greater fidelity than with Taq DNA polymerase
- Amplifying fragments of up to 5 kb
Contents:
Biotools Pfu DNA Polymerase is supplied with the choice of 10 X Standard Reaction Buffer including 2 mM MgCl2 or a reaction buffer without MgCl2 (10 X Reaction Buffer MgCl2 Free) plus a separate tube of MgCl2 for optimising the magnesium concentration.
Specifications:
- Polymerase: Pfu
- Hot Start effect: No
- Fidelity (vs Taq): ++++
- Concentration: 1 U/μl
- GC-Rich PCR Performance: No
- Reaction Format: Separate components
- Amplification size: Up to 5 kb
- Product size: 100 U, 250 U
- Exonuclease Activity: 3’ - 5’
- Shipping condition: Cold shipping
- Storage condition: -20ºC
- Regulatory Statement: For research use only. Not for use in diagnostic procedures
