DNA AmpliGel Master Mix Strips

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The process is simple because all the reagents needed for the PCR reaction are included in each vial, so just add the sample (DNA and primers).
DNA AmpliGel Master Mix Strips

Biotools has developed a new PCR product using one of its international patents: GELIFICATION. The process is simple because all the reagents needed for the PCR reaction are included in each vial, so just add the sample (DNA and primers). 

FEATURES

READY-TO-USE 

  • One well- One reaction format.
  • Ready for in-field manipulation.
  • No optimization needed.

LESS MANIPULATION & LESS CONTAMINATION

  • Only need to add primers and DNA sample.
  • Customized plates available: Give us your primers and we will include them in the Gelified reaction.

SHIPMENT AT ROOM TEMPERATURE AND STORAGE AT 4ºC

Stable for long time periods at 4ºC.

HOT-START EFFECT

Reaction starts when the Gel is completely rehydrated with the increase of temperature.

NO NEED TO RESUSPEND

Reaction will become liquid automatically with the increase of temperature (no handling).

ADVANTAGES vs LYOPHILIZATION

  • Components keep their activity.
  • No need to resuspend. 
  • Final concentration is mantained.

DNA AmpliGel Master Mix Strips - (12 x 8-Strips) and the gelified mix reagents can be stored at 4 °C respectively for long periods of time. The Ready-to-Use format minimises risk of contaminations, while promoting rapid sample processing ideal for routine applications. 

This enzyme is a recombinant DNA Polymerase from Thermus sp. and cloned in E. coli, with both 5'-3' polymerase and 5'-3' exonuclease activities, and reduced error rates (1 - 10x 10-6). 

Thermostable enzyme perfect for routine amplifications including:

  • Amplification of PCR products of up to 5 Kb 
  • Colony screening 
  • Recombinant cDNA construction 
  • Genotyping 
  • Routine DNA amplifications 

DNA AmpliGel Master Mix Strips - (12 x 8-Strips) is available in Vials (fv 25 µl) and in 96 well Plates (fv 25 µl). 

Contents:

DNA AmpliGel Master Mix Strips - (12 x 8-Strips) 96 well Plates is supplied with 10 X Standard Reaction Buffer including 2 mM MgCl2and dNTPs. 

DNA AmpliGel Master Mix Strips - (12 x 8-Strips) Vials (0,2 vials) 96 vials. Each vial includes Standard Reaction Buffer with 2 mM MgCl2, dNTPs, and DNA polymerase.

Specifications:

  • Master Mix: DNA AmpliGel Master Mix Strips
  • Hot Start effect: Yes
  • GC-Rich PCR Performance: No
  • Reaction Format: Gelified format and Ready to use
  • Amplification size: Up to 5 kb
  • Product size: 96 vials
  • Shipping condition: Room temperature
  • Storage condition: 4ºC
  • Regulatory Statement: For research use only. Not for use in diagnostic procedures

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