High Scriptools OneStep Kit

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High Scriptools OneStep Kit is a novel RT-PCR system for the detection of specific sequences within an RNA sample.
High Scriptools OneStep Kit

This one-step, two-enzyme system uses High Retrotranscriptase and Biotools HotSplit DNA Polymerase for detection of any type of RNA sample.
 
The kit has been designed to deliver maximum efficiency, precision and sensitivity of RT-PCR. For these purpose two high-performance enzymes, a thermostable reverse transcriptase and a DNA polymerase with hot start activity, carry out the reactions. Both cDNA synthesis and PCR are performed sequentially in a single tube due to a single buffer system formulated to ensure specific primer annealing.

High Scriptools OneStep Kit provides a convenient and fast procedure, one step only, for synthesizing cDNA and DNA amplification. All reagents necessary for both reactions are added in one tube at the same time which confers a rapid and easy-to-handle format saving manipulation time and reducing contamination risks without compromising efficiency or sensitivity of the kit. To use it, simply add to a vial containing the prepared Master Mix, desired primers, and RNA template.

REAGENTS INCLUDED IN THE KIT

The system contains sufficient reagent for a number of one-tube RT-PCR reactions of 50µl each.

  • High Master Mix: An easy and convenient 2X Master Mix formulated to setting up one-tube RT-PCR. The mixture includes: Biotools HotSplit DNA Polymerase, dNTPs, MgSO4, and Reaction Buffer.
  • High Retrotranscriptase: A new RNase H minus, thermostable reverse transcriptase which exhibits high affinity for RNA and works in a higher range of temperature than most commercially retrotranscriptases. The RT enzyme is provided in a separate tube.
  • PCR Astringent: Used for specific RT-PCR assays, to increase sensitivity and specificity of RT-PCRs.
  • 100 mM MgSO4 Solution: Used only for assays which require an additional optimisation.

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