Biotools High Retrotranscriptase

The enzyme is an RNase H minus which exhibits high affinity for RNA and works in a higher range of temperature than most commercially retrotranscriptases. Due to its thermostability, Biotools High Retrotranscriptase can transcribe GC-rich templates with large amounts of secondary structure without the help of reaction additives.

Biotools High Retrotranscriptase will accept three different types of primer for cDNA synthesis: Oligo (dT) primer; random hexamer primers; or a gene-specific primer. The enzyme can be used at a temperature up to 65°C, and it is recommended for RT-PCR or qRT-PCR.

Product applications:

• Synthesis of first strand cDNA for use in subsequent amplification reactions: RT-PCR or qRT-PCR.
• RT-PCR amplification of difficult RNA templates, such as GC-rich templates with large amounts of secondary structure.
• Incorporation of Cy3-, Cy5-, Biotin-, or aminoallyl-modified nucleotides during cDNA synthesis (e.g., for use in microarray hybridization).

REAGENTS INCLUDED IN THE KIT

• Biotools High Retrotranscriptase: Provided in storage buffer. Storage buffer: 5 mM Tris-Acetate (pH 8.0), 15 mM KCl, 1.5 mM EDTA, protease inhibitors, and 50% glycerol (v/v).
• 10X High RT Reaction Buffer: 100 mM Tris-HCl (pH 9.0), 500 mM KCl, and 1 mM DTT.
• 100 mM MgSO₄ Solution.