MHC I-Strep HLA-A*0201; FMOD F1 (LLLAGLFSL)

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MHC class I molecule fused to a Twin-Strep-tag® and refolded with a peptide
MHC I-Strep HLA-A*0201; FMOD F1 (LLLAGLFSL)

MHC I-Strep HLA-A*0201; Fibromodulin F1(7-17) (LLLAGLFSL) is a recombinantly expressed MHC class I molecule that presents a peptide from a tumor-associated antigen Fibromodulin F1. Our MHC I Streptamer® approach is based on our Strep-tag® technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag® fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin® backbone, resulting in the formation of MHC I Streptamers.

For multimerizing MHC I-Streps, Strep-Tactin® TACS Agarose columns, Strep-Tactin® Magnetic Microbeads or fluorophore-conjugated Strep-Tactin® can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements. The addition of biotin reverses the multimerization of MHC class I molecules, leading to their dissociation from the target cells. The resulting label-free cells are highly viable and fully functional.

Specifications

  • Concentration: See data sheet for specific Lot. No.
  • Form: Dissolved in DPBS
  • Peptide Sequence: LLLAGLFSL
  • Possible Application: Fluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolation
  • Recommended Usage: 0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolation
  • Sterility: Sterile
  • MHC I Allele: HLA-A*0201

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